Carboxyfluorescein FLICA Assay Kits

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FLICA Apoptosis Detection Kits use a novel approach to detect active caspases. The method is based on a Fluorochrome Inhibitor of Caspases (FLICA).  Once inside the cell, the FLICA inhibitor binds covalently to the active caspase.  These inhibitors are cell permeable and non-toxic.  For detection using green fluorescence, a carboxyfluorescein-labeled fluoromethyl ketone peptide inhibitor of caspases is used.
 

Photo A, only one cell appears green - it is apoptotic and stained positive for poly caspase activity with FAM-VAD-FMK reagent.  The other cell, which is not visible, did not bind to the reagent and therefore not apoptotic.  The same cells, photographed at right under a different wavelength for Hoechst stain appear blue.  The cell in the top right of photo B (which appears green in photo A) has a very bright nucleus - its DNA is condensing in the cell, a sign it is dying.

 

Carboxyfluorescein FLICA Kits

 

Product Peptide  Size Catalogue Number
FAM Poly Caspase Assay Kit FAM-VAD-FMK 25 tests BB91
FAM Poly Caspase Assay Kit FAM-VAD-FMK 100 tests BB92
FAM Caspase-1 Assay Kit FAM-YVAD-FMK 25 tests BB97
FAM Caspase-1 Assay Kit FAM-YFVAD-FMK 100 tests BB98
FAM Caspase-2 Assay Kit FAM-VDVAD-FMK 25 tests BB918
FAM Caspase-2 Assay Kit FAM-VDVAD-FMK 100 tests BB919
FAM Caspase-3 & 7 Assay Kit FAM-DEVD-FMK 25 tests BB93
FAM Caspase-3 & & Assay Kit FAM-DEVD-FMK 100 tests BB94
FAM Caspase-6 Assay Kit FAM-VEID-FMK 25 tests BB95
FAM Caspase-6 Assay Kit FAM-VEID-FMK 100 tests BB96
FAM Caspase-8 Assay Kit FAM-LETD-FMK 25 tests BB99
FAM Caspase-8 Assay Kit FAM-LETD-FMK 100 tests BB910
FAM Caspase-9 Assay Kit FAM-LEHD-FMK 25 tests BB912
FAM Caspase-9 Assay Kit FAM-LEHD-FMK 100 tests BB913
FAM Caspase-10 Assay Kit FAM-AEVD-FMK 25 tests BB922
FAM Caspase-10 Assay Kit FAM-AEVD-FMK 100 tests BB923
FAM Caspase-13 Assay Kit FAM-LEED-FMK 25 tests BB929
FAM-Caspase-13 Assay Kit FAM-LEED-FMK 100 tests BB930

For Research Use Only

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Metachem Diagnostics Ltd.
29 Forest Road, Piddington, Northampton. NN7 2DA
Tel. (01604) 870370     Fax (01604) 870194