Cytogenetics  Media  


     Home    Back

AmnioSelect is a ready to use medium and has been specifically developed for the prenatal diagnostic testing of human amnion and chorionic villi cells. The cultivation of these cells and the preparation of karyograms allow the detection of possible chromosomal aberrations.  The medium is supplied frozen and contains serum L-glutamine and antibiotics. 

LymphoSelect medium is intended for use in short-term cultivation of peripheral blood lymphocytes for chromosome evaluation.  The medium is base on RPMI 1640 basal medium supplemented with L-Glutamine, feotal bovine serum, antibiotics (gentamicin) and phytoheagglutinin-M (PHA-M).  It is supplied as frozen medium that is ready for use after thawing.

Colcemid Solution: N-Deacetyl-N-methylcolchicine (Colcemid) is related to colchicine, but it is less toxic.  Colcemid depolymerises microtubles and limits their formation.  Appropriate dilutions inactivate the spindle fiber formation thus arresting cells in metaphase and allowing cell harvest and karyotyping to be performed.

Phytohemagglutinin is a lectin extracted from red kidney beans (Phaseolus vulgaris).  The protein consists of two molecular species, a leucoagglutinin (PHA-L) and an erythroagglutinin (PHA-E).  Each of the proteins contains a family of five isolectins, each being a tetramer held together by noncovalent forces.  PHA-M is the mucoprotein form and is a crude extract used for the stimulation of cell proliferation in lymphocyte culture.  PHA-M also has a powerful erythroagglutinating propery and it was originally used for separating leukocytes from whole blood.  PHA-M is a sterile, frozen solution of an aqueous extract from selected red kidney beans.

Catalogue Number

Description

Size

Tri-21B

AmnioSelect

                        100 mL

PBL-1B

LymphoSelect

100 mL

COL-H

Colcemid Solution (10mg/mL) in DPBS

10 mL

PHK-K

Phytohemagglutinin M (PHA-M)

5 mL

 

     Home     Back

Metachem Diagnostics Ltd. 29 Forest Road, Piddington, Northampton. NN7 2DA.
Tel. (01604) 870370     Fax (01604) 870194