Anti-sera 


Manufactured by B-Bridge International Inc

  

   

Anti-HIV-1 p24 antibody

Immunized Animal: Guinea Pig

Polyclonal antiserum

Cat. # 65-006 (50 μl)

Description

HIV-1 Gag p24 is a capsid protein that constitutes the core of AIDS virus HIV-1 and is produced by the digestion of its precursor Gag p55 by HIV-1 protease. This protein is indispensable to the reproduction of AIDS virus and constitutes an essential element for the AIDS virus particle construction (1). As this protein is detectable from the early stage of AIDS virus infection, it is used as a marker for observation of the development in the patient’s condition after treatment, as it indicated the amount of virus in the blood.

The product is prepared by immunizing Guinea Pig with recombinant p24 protein which was over-expressed in E. coli with a plasmid carrying the Gag p24 coding region of HIV-1 virus, subtype B (2), and highly purified by several steps of chromatography (3, 4).

Using this antiserum in Western blotting, the bands of 24 kD, 55 kD and 41 kD corresponding respectively to HIV-p24 and to its precursors p55 and p41 were observed in the extract of the AIDS virus infected cells (Fig. 1).

Usage

Use for Western blotting or ELISA for HIV-1 p24 detection and titration.

Specification

Form: 0.09% sodium azide added to the antiserum.
Storage: 4
oC

References

1. Freed EO, Virology 251:1-15 (1998) Review
2. Adachi A, et al., J. Virol. 59, 284 (1986)
3. Tanaka N, et al., Microbiol. Immunol. 36:823-831 (1992)
4. Saito A, et al., Microbiol. Immunol. 39:473-483 (1995)

Fig. 1: Detection of HIV-1 p24 and precursor proteins p55 and p 41 by Western blotting using the p24 antibody. 
           Lane 1, Extract of MT4 cells.
           Lane 2, Extract of MT4 cells infected with HIV-1(LAI strain). 
           The antiserum was diluted 2500 fold before use.

                                                                                                   Ver. 092006anti-hiv-1p24

For in vitro use only

  

Metachem Diagnostics Ltd. 29 Forest Road, Piddington, Northampton. NN7 2DA.
Tel. (01604) 870370     Fax (01604) 870194