Anti-sera 


Manufactured by B-Bridge International Inc

  

   

Anti-HIV-1 Reverse Transcriptase Antibody

Immunized Animal: Rabbit

Polyclonal antiserum

Cat. # 65-002 (250 μl)

Description

HIV-1 reverse transcriptase is an RNA-dependent DNA polymerase of HIV-1(AIDS virus), subtype B origin (1). It also has RNaseH activity and is an enzyme indispensable to the reproduction of AIDS virus.

The product is prepared by immunizing rabbit with HIV-1 reverse transcriptase which was uniquely over-overexpressed as recombinant protein in E. coli by a patent method and highly purified (2).

Using this antiserum in Western blotting, the bands of 66 kD and 51 kD corresponding respectively to the two subunits of HIV-1 reverse transcriptase were observed in the extract of the AIDS virus infected cells (Fig 1).

Usage

It can be used in Western blotting or ELISA for HIV-1 reverse transcriptase detection and titration.

Specification

Form: 0.09% sodium azide added to the antiserum.
Storage: 4
oC

Reference

1. Adachi A, et al., J. Virol. 59, 284 (1986)

2. Saitoh A, et al., Microbiol. Immunol. 34:509-521 (1990)

   


Fig. 1
:
Detection of HIV-1 reverse transcriptase by Western blotting using anti-HIV-1 reverse transcriptase antibody.
 Lane 1, extract of MT4 cells. Lane 2, extract of MT4 cells infected with HIV-1 (LAI strain). 
The antiserum was diluted 2500 fold before use.

                                                                                                                              Ver. 092006anti-hiv-1RT

 

For in vitro use only

  

Metachem Diagnostics Ltd. 29 Forest Road, Piddington, Northampton. NN7 2DA.
Tel. (01604) 870370     Fax (01604) 870194