by B-Bridge International
Obesity, and obesity related disorders, are reaching alarming proportions in the US, and are on the increase in Europe and Asia. A deeper understanding of the molecular and cellular dynamics of such disorders, and their subsequent amelioration, will have a far-reaching impact on the quality of life of millions of people worldwide.
Adipocytes (fat cells) express a variety of proteins that function in the homeostatic control of glucose and lipid metabolism. Insulin regulates the translocation and secretion of many of these proteins in response to changes in energy balance. Adipocyte complement-related protein of 30 kDa (Acrp30), now known as adiponectin, is a protein whose secretion from adipocytes is enhanced by insulin stimulation.
It has been suggested that the development of non-insulin dependent (Type 11) diabetes may involve dysregulation of adiponectin secretion. In support of the link between obesity and Type 11 diabetes, it has been shown that decreased expression of adiponectin correlates with insulin resistance, and that adiponectin appears to be a potent insulin enhancer linking adipose tissue and whole-body glucose metabolism.
The B-Bridge Mouse/Rat Adiponection ELISA kit is designed to measure the concentration of human adiponectin from human serum/plasma, human adipocytes, or conditioned medium.
The principle of the assay: Samples and serially diluted standard (recombinant mouse adiponectin) solutions are added to an appropriate number of wells of the microtitre plate and incubated. Adiponection in the sample will be bound by the primary anti-mouse adiponectin polyclonal antibody immobilised in the well (1st Reaction). After washing, the biotinylated secondary rabbit anti-mouse adiponectin antibody is added to each well and allowed to incubate (2nd Reaction). The biotinylated secondary rabbit anti-mouse adiponectin polyclonal antibody will bind to the adiponection trapped in the well in the 1st Reaction. After washing, a conjugate of horseradish peroxidase (HRP) and streptavidin is added to each well and allowed to incubate (3rd Reaction). The HRP-cojugated streptavidin will recognise and bind to the biotinylated rabbit anti-adiponection antibody trapped in the well in the 2nd Reaction. After washing, the colorimetric substrate for the enzyme is added to all wells and incubated. The colour development if terminated by the addition of a stop solution. The intensity of the colour is measured at 450 nm. The concentrations of the samples tested are calculated using the absorbance values of the adiponection standard solutions assayed at he same time.
Mouse/Rat Adiponectin ELISA Kit
|Colorimetric (EIA) Kits||Catalogue Number|
96 well plate
adiponectin polyclonal Ab displays detection levels outside the assay
range with other species.
FOR RESEARCH USE ONLY - NOT FOR USE IN DIAGNOSTIC PROCEDURES
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