Anti-sera 


Manufactured by B-Bridge International Inc

  

    

Anti-Rad22 (S. pombe) antibody (rabbit serum)

Immunized Animal: Rabbit

Polyclonal antiserum

Cat. No. BB63-003, 50 mL

Rad22 protein of Schizosaccharomyces pombe (fission yeast) is a functional and structural homolog of S. cerevisiae and human Rad52 proteins, which play a major role in genetic recombination and recombination repair by mediating strand annealing reaction between homologous DNA strands (1).

The product was prepared by immunizing rabbit with recombinant Rhp22 protein expressed in E. coli and purified. Using this anti-serum, Rad22 protein (52 kD) in the crude extract was detected by Western blotting at the position near the 60 kD marker (Fig 1)

Applications

1) Western blotting (x 2,000~5,000* dilution)

2) Immuno-precipitation

3) Detection of foci formation by indirect immunofluorescence

Specification

Form; 0.09 % sodium azide added

Storage: 4 C

Reference

1.Lehmann AR, Mutation Res. 363, 147 (1996) Review

Fig. 1: Western blot analysis of Rad22 in whole cell extracts.

M: Molecular weight markers (kD)

Lane 1: Wild-type strain.

Lane 2: rad22 deletion strain.    

* May vary among different lot numbers.                                      

 

For in vitro use only

  

Metachem Diagnostics Ltd. 29 Forest Road, Piddington, Northampton. NN7 2DA.
Tel. (01604) 870370     Fax (01604) 870194