Anti-sera 


Manufactured by B-Bridge International Inc

  

    

Anti-Rhp51 (fission yeast) rabbit serum

Immunized Animal; Rabbit

Polyclonal antiserum

Cat. No. 63-002, 250 μL

Description

Rhp51 protein of Schizosaccharomyces pombe (fission yeast) is a functional and structural homolog of E. coli RecA protein and the Rad51 proteins of eukaryotes. These proteins play a major role in genetic recombination and recombination repair by mediating strand exchange reaction between homologous DNA strands (1).
The product was prepared by immunizing rabbit with full-size recombinant Rhp51 protein expressed
in E. coli and purified.
Using this anti-serum, the Rhp51 protein (40 kD) in the crude extract was detected by Westernblotting (Fig 1) and Rhp51 foci formation, induced by stalled replication (2) and DNA-damage (3), was
detected by indirect immunofluorescence. GFP-tagging of Rhp51 protein at either N- or C- terminus inactivates the function of Rhp51, and therefore, cannot be used for the study of foci formation instead of the antibody. This antibody was also successfully used for immunoprecipitation (4).

Usage

 Western blotting (x2,000~5,000*dilution)

 Immunoprecipitation

 Detection of foci formation by indirect immunofluorescence

Specifications

Formulation: Serum in 0.09% sodium azide

Storage: 40C (long term: -700C)

Reference

1.Lehmann AR, Mutation Res. 363, 147 (1996) Review

2. Lambert S, et al., Cell 121, 689 (2005)

3. Morishita T, et al., Mol. Cell. Biol. 25, 8074 (2005)

4. Haruta N. et al, Nat. Struc. Mol. Biol. 13, 823(2006)

Fig. 1: Western blot analysis of Rhp51 in whole cell extracts.

M: Molecular weight marker (kD)

Lane 1: Wild-type strain

Lane 2: rhp51 deletion strain.

                                                                        

* May vary among different lot numbers.

 

For in vitro use only

  

Metachem Diagnostics Ltd. 29 Forest Road, Piddington, Northampton. NN7 2DA.
Tel. (01604) 870370     Fax (01604) 870194