Manufactured by B-Bridge International Inc
Anti-Rhp51 (fission yeast) rabbit serum
Immunized Animal; Rabbit
No. 63-002, 250 μL
of Schizosaccharomyces pombe (fission yeast) is a functional and structural
homolog of E.
coli RecA protein and the Rad51
proteins of eukaryotes. These proteins play a major role in genetic
recombination and recombination repair by mediating strand exchange reaction
between homologous DNA strands (1).
The product was prepared by immunizing rabbit with full-size recombinant Rhp51 protein expressed in E. coli and purified.
Using this anti-serum, the Rhp51 protein (40 kD) in the crude extract was detected by Westernblotting (Fig 1) and Rhp51 foci formation, induced by stalled replication (2) and DNA-damage (3), was detected by indirect immunofluorescence. GFP-tagging of Rhp51 protein at either N- or C- terminus inactivates the function of Rhp51, and therefore, cannot be used for the study of foci formation instead of the antibody. This antibody was also successfully used for immunoprecipitation (4).
UsageWestern blotting (x2,000~5,000*dilution) Immunoprecipitation
Detection of foci formation by indirect immunofluorescence
Formulation: Serum in 0.09% sodium azide
Storage: 40C (long term: -700C)
1.Lehmann AR, Mutation Res. 363, 147 (1996) Review
2. Lambert S, et al., Cell 121, 689 (2005)
3. Morishita T, et al., Mol. Cell. Biol. 25, 8074 (2005)
4. Haruta N. et al, Nat. Struc. Mol. Biol. 13, 823(2006)
Fig. 1: Western blot analysis of Rhp51 in whole cell extracts.
M: Molecular weight marker (kD)
Lane 1: Wild-type strain
Lane 2: rhp51 deletion strain.
* May vary among different lot numbers.
For in vitro use only
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