Anti-sera 


Manufactured by B-Bridge International Inc

  

    

Anti-Srb4p antibody

Immunized Animal: Rabbit

Polyclonal antiserum

Cat. # 62-007 (50 μl)

Description

Mediator is a protein complex which performs a very important role both for the transcription at the basal level which does not rely on transcription control factor and the transcription activating reaction that relies on the transcription control factor. It has the characteristic of binding to the 7 amino acid repeated structure (CTD) that exists in the C terminal of the biggest subunit of RNA polymerase II. 30 kinds of subunits have already being identified as its composing substance, but it is said that multiple kinds of complex with varied subunit compositions exist in the cells. Srb4p is one of the mediator subunit and is composed of 687 amino acid residues (aa).

The product is prepared by immunizing rabbit with recombinant protein which was over-expressed in E. coli with a plasmid carrying the entire Srb4p protein (1-687aa) of budding yeast, and purified by chromatography.

Using this antiserum in Western blotting, the band of 100 kD corresponding to Srb4p was obtained from the extract of yeast cells (Fig. 1).

Applications

It can be used in Western blotting or ELISA for the detection and titration of budding yeast Srb4p.

Specifications

Form: 0.1% sodium azide added to the antiserum.                                    
Storage: 4
oC

                                                                                                                                                                           

Fig. 1: Detection of Srb4p by Western blotting using the Srb4p antibody.
             Lane 1, Extract of budding yeast.
             The antiserum was diluted 5000 fold before use.

                                                                                                                                  Ver. 092006AntiSrb4p

For in vitro use only

  

Metachem Diagnostics Ltd. 29 Forest Road, Piddington, Northampton. NN7 2DA.
Tel. (01604) 870370     Fax (01604) 870194