Sulforhodamine FLICA Assay Kits
FLICA Apoptosis Detection Kits use a novel approach to detect active caspases. The method is based on a Fluorochrome Inhibitor of Caspases (FLICA). Once inside the cell, the FLICA inhibitor binds covalently to the active caspase. These inhibitors are cell permeable and non-toxic. For detection using red fluorescence, a sulforhodamine-labeled fluoromethyl ketone peptide inhibitor of caspases is used.
cells, grown in suspension, were incubated with 1mM staurosporine for 3 hours at
to induce apoptosis. Cells were then labeled with SR-VAD-FMK for 60 minutes at 37 0C. Slides
were prepared and samples viewed through a fluorescence microscope using a broad band filter.
Sulforhodamine FLICA Kits
|Sulforhodamine Poly Caspase Detection Kit||SR-VAD-FMK||25 tests||BB916|
|Sulforhodamine Poly Caspase Detection Kit||SR-VAD-FMK||100 tests||BB917|
|Sulforhodamine Caspase-3 & &Assay Kit||SR-DEVD-FMK||25 tests||BB931|
|Sulforhodamine Caspase-3 & 7 Assay Kit||SR-DEVD-FMK||100 tests||BB932|
|Sulforhodamine Caspase 9 Assay Kit||SR-LEHD-FMK||25 tests||BB960|
|Sulforhodamine Caspase 9 Assy Kit||SR-LEHD-FMK||100 tests||BB961|
For Research Use Only
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Tel. (01604) 870370 Fax (01604) 870194