Manufactured by B-Bridge International Inc
E. coli RecA protein plays critically important roles in homologous recombination, recombination repair and regulation of cellular responses to DNA damage (SOS response). RecA promotes auto-cleavage of LexA repressor by its co-protease activity after DNA damage, and induces many proteins related to DNA repair, including RecA itself (1).
The product was prepared by immunizing rabbit with the recombinant protein, which was over-expressed in E. coli, and highly purified by chromatography.
Using this antiserum in Western blotting, the band of 38 kD corresponding to RecA was obtained from the extract of E. coli cells (Fig. 1).
?It can be used in Western blotting or ELISA for the detection and titration of E. coli RecA protein for the studies of SOS regulation (2, 3). For western blotting, use the serum at ~1,000 dilution.
?Since RecA has many functions and interacts with various proteins in the cell, the protein complexes can be detected by immunoprecipitaion with the anti-RecA antibody.
?RecA forms foci on nucleoids in the cells after exposure to DNA damaging treatments. The foci is visualized by immuno-staining of the cells using this antibody as the first antibody.
Formulation: 0.05% sodium azide added to antiserum
Storage: 4℃ for short period (~a half year). For longer periods, store at 80℃
1. Friedberg EC, et al. DNA Repair and Mutagenesis 2nd ed., ASM Press (2005)
2. Hishida T, et al., Genes Dev. 18, 1886-1897 (2004)
3. Shibata T, et al. Genes Cells 10, 181-191 (2005)
Detection of Gal11p by Western
blotting using the Gal11p antibody.
Lane 1: Extract of budding yeast.
The antiserum was diluted 5000 fold before use.
For in vitro use only
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