Anti-sera 


Manufactured by B-Bridge International Inc

  

    

 Anti-E.coli LexA antibody
 
Immunised Animal: Rabbit  
 
Polyclonal antiserum
  BB61-02 (250 mL)

E. coli LexA protein binds specifically to the SOS-box sequence and represses the genes belonging to the SOS regulon. In response to DNA damage, RecA protein is activated by ss-DNA accumulated in the damaged cells and promotes autocleavage of LexA repressor by its coprotease activity. As the results, DNA repair genes and error prone polymerases are induced, and DNA damage is repaired and mutation is induced (1). The lexA gene is used for yeast two-hybrid experiments as a “bait” to identify the protein-protein

interaction in vivo (2).

This product was prepared by immunizing rabbit with full-size highly-purified recombinant LexA protein. Using this antibody, 23 kD LexA protein was identified in the E.coli whole-cell lysate (Fig 1) and the expression of bait constructs was identified in yeast extracts by western blotting.

Applications

1) Studies on the SOS regulation in E.coli (3). For western blotting; 1000~3000* fold dilution.
2) Construction and expression of a bait protein fused to LexA protein can be examined by western
blotting of the yeast extracts,using the antiserum.

Purified LexA protein is available from B-Bridge (#01-002) to be used as a positive control for western blotting.

Specifications

Form: antiserum added with 0.05% sodium azide.

Storage: 4°C (long term,–70°C).

Reference:

1. Friedberg EC, et al. DNA Repair and Mutagenesis 2nd Ed., ASM Presss (2005)

2. Sambrook J & Russell DW, Molecular Cloning 3rd Ed. Cold Harbor Spring Press (2001)                        

3.Hishida T, et al., Genes Dev. 18, 1886-1897 (2004)

 

Fig.1 Detection of LexA repressor in the whole cell lysate of E. coli.

* May vary among different lot numbers.  

                                                                                                          

 

 

 

                                                                                                        
           
             

                                                                                                                                  

 

For in vitro use only

  

Metachem Diagnostics Ltd. 29 Forest Road, Piddington, Northampton. NN7 2DA.
Tel. (01604) 870370     Fax (01604) 870194