Human Resistin


Manufactured by B-Bridge International

       Home    Immunoassays

Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins which are termed the RELM family, and is also describes as ADSF (Adipose Tissue-Specific Secretory Factor) and FIZZ3 (Found in Inflammatory Zone).  Human resistin contains 108 amino acids as a pre-peptide, its hydrophobic signal peptide is cleaved before it is secreted.  Resistin circulates in human blood as a dimeric protein consisting of two 92 amino acid polypeptides, which are disulfide-linked via Cys26.

Resistin may be an important link between obesity and insulin resistance.  Mouse resistin, specifically produced and secreted by adipocytes, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves to reduce their sensitivity to insulin.  Steppan et al (2001) have suggested that resistin suppresses the ability of insulin to stimulate glucose uptake.  They also suggested that resistin is present at elevated levels in blood of obese mice, and is down regulated by fasting and antidiabetic drugs.  Way et al (2001), on the other hand, have found that resistin expression is severely suppressed in obesity and is stimulated by several antidiabetic drugs.  Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it aslo seems to inhibit adipogenesis.  In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression.  

The B-Bridge Human Resistin ELISA Kit is designed to measure the concentration of human resistin in serum, plasma or tissue culture medium.

 

             Well Correlated Linearity

 

Several dilution series were run with  human resistin. As you can see, each  dilution results in a linear and well- correlated  decrease in absorbance.

B-Bridge's Human Resistin ELISA Kits robustly detect as little as 0.2 ng/ml of human  resistin in serum, plasma, and culture medium. With intra-assay CVs below 5% and inter-assay CVs below 6%, the biotin-labeled antibody-based sandwich enzyme immunoassay provides reproducible results over a broad dynamic range from assay to assay. The antibodies in Human Resistin ELISA Kits are highly specific for human resistin with no detectable cross-reactivities to mouse resistin and other cytokines that may be present in human serum. 

 Human Resistin ELISA Kit

Colorimetric (EIA) Kits Catalogue Number
96 well plate K1003-1

Click on logo to visit B-Bridge International Website

       Home   Immunoassays

Metachem Diagnostics Ltd. 29 Forest Road, Piddington, Northampton. NN7 2DA.
Tel. (01604) 870370     Fax (01604) 870194